Beef Heart Malic Dehydrogenases
نویسندگان
چکیده
منابع مشابه
Beef heart malic dehydrogenases. VII. Reactivity of sulfhydryl groups and conformation of the supernatant enzyme.
Only 3 of the 6 sulfhydryl groups of native bovine heart supernatant malate dehydrogenase (S-MDH) react with p-mercuribenzoate (PMB) with no loss of enzymatic activity. In addition, prolonged incubation of S-MDH in 2.6 M urea solutions does not significantly alter the catalytic properties of the enzyme. In 2.6 M urea, however, all 6 sulfhydryl groups of the enzyme react with PMB, with attendant...
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A study of the activity and distribution of glutamic dehydrogenase in various tissues of the developing chick embryo has recently been made (Solomon, 1957). It was found that glutamic dehydrogenase showed marked fluctuations of activity in certain tissues at various stages of development. However, as only relatively low amounts of the enzyme were found in all tissues of the chick embryo and fow...
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Lactic dehydrogenase has been shown to exist in mulbiple molecular forms within a single tissue in several different species (2-15). It is now fairly well established that the lactic dehydrogenase molecule is a tetramer composed of four subunits and that there are two types of these subunits (16, 17). The subunits may combine in all possible combinations of four to yield five different tetramer...
متن کاملMultiple Molecular Forms of Malic and Lactic Dehydrogenases during Development*
Lactic dehydrogenase has been shown to exist in mulbiple molecular forms within a single tissue in several different species (2-15). It is now fairly well established that the lactic dehydrogenase molecule is a tetramer composed of four subunits and that there are two types of these subunits (16, 17). The subunits may combine in all possible combinations of four to yield five different tetramer...
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The effects of methotrexate (MTX) on oxygen uptake by permeabilized HeLa cells were evaluated. MTX did not inhibit state III respiration when the oxidizable substrate was succinate, but when the substrates were 2-oxoglutarate or isocitrate the respiration decreased about 50 per cent at 1.0 mM concentration of the drug. This effect was explained by inhibition of 2-oxoglutarate and isocitrate deh...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96834-1